AM009 – L-Kynurenine antibody

Monoclonal anti-conjugated L-Kynurenine antibody (mouse)

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Specifity

Using L-Kynurenine -BSA conjugate (KYNU-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

L-Kynurenine-BSA Kd around 10-8 M very high affinity and specificity

Low affinity or no crossreactivity with close competitors:

Competitors Affinity compared to KYNU-BSA
D-Kynurenine-BSA 7 times lower high affinity
Picolinic Acid-PC 3 000 times lower low affinity
Kynurenic acid-BSA 50 000 times lower no crossreactivity
3-Hydroxy-Kynurenine-BSA 50 000 times lower no crossreactivity
Xanthurenic acid-BSA 50 000 times lower no crossreactivity
Quinaldic acid-BSA 50 000 times lower no crossreactivity
L-Tryptophan-BSA 50 000 times lower no crossreactivity
Quinolinic acid-BSA 50 000 times lower no crossreactivity
Anthranilic acid-BSA 50 000 times lower no crossreactivity
L-kynurenine free 50 000 times lower no crossreactivity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Description

Monoclonal antibody was obtained after BALB/c mouse immunisation with the conjugate: L-Kynurenine-bovine serum albumin (BSA). Hybridoma clone was obtained by hybridization of spleen cells with the myeloma cell line SP2/O/Ag14

Host species

Mouse

Clonality

Monoclonal

Clone number

KYNU4A6

Sub-categories

IDO compounds

Isotype

IgG1, Kappa

Target

Conjugated L-Kynurenine

Immunogen

Synthetic L-Kynurenine conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule

Purity

Monoclonal antibody was preadsorbed on protein carriers and purified by ammonium sulfate precipitation

Form

Lyophilized

Stabilizers / preservatives

None

Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Neurobiology, Neurodegenerative diseases

Applications

ELISA, Immunohistochemistry, Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Gemacbio sells as well the corresponding antigen: L-Kynurenine conjugate (Product number: AG147)

Related antibodies

Gemacbio also sells the polyclonal anti-L-Kynurenine antibody (AP147). Gemacbio sells as well polyclonal antibodies against other small molecules of the IDO pathway: anti-Kynurenic acid (AP062), anti-Quinolinic acid (AP063)