AP006 – Noradrenaline (Norepinephrine) antibody

Polyclonal anti-conjugated Noradrenaline (Norepinephrine) antibody (rabbit)

From 180

Clear

Additional information

Specifity

Using Noradrenaline-BSA conjugate (NA-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

Noradrenaline-BSA Kd around 10-8M very high affinity and specificity

Very low affinity with close competitors:

Competitors Affinity compared to NA-BSA
Octopamine-BSA 30 times lower low affinity
Dopamine-BSA 70 times lower low affinity
Adrenaline-BSA 180 times lower low affinity
L-DOPA-BSA 5 000 times lower very low affinity
Tyramine-BSA 5 000 times lower very low affinity
Noradrenaline free 5 000 times lower very low affinity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Host species

Rabbit

Clonality

Polyclonal

Sub-categories

Catecholamines

Isotype

IgG

Target

Conjugated Noradrenaline

Immunogen

Synthetic Noradrenaline conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule

Purity

Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation

Form

Lyophilized

Stabilizers / preservatives

None

Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Cardiovascular research, Neurobiology, Neurodegenerative diseases, Pharmacology

Applications

ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate your immunolabelling and the specificity of the response in your biological samples, Gemacbio sells as well the corresponding antigen: Noradrenaline conjugate (Product number: AG006)

Related antibodies

Gemacbio also sells antibodies against small molecules of the Phenylalanine pathway: anti-L-DOPA (AP004), anti-Dopamine (AP001 or AM001), anti-adrenaline (AP005), anti-Octopamine (AP007), anti-Tyramine (AP008), anti-Tyrosine (AP010), anti-Phenylalanine (AP039). Gemacbio sells as well antibodies against other neurotransmitters: anti-Serotonin (AP014), anti-GABA (AP027), anti-Glycine (AP028), anti-L-Glutamate (AP029), anti-Acetylcholine (AP049 or AM002), anti-Histamine (AP032 or AM005)