AP004 – L-DOPA antibody

Polyclonal anti-conjugated L-DOPA antibody (rabbbit)

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Additional information

Specifity

Using L-DOPA-BSA conjugate, antibody specificity was performed by using an ELISA test by competition experiments:

L-DOPA-BSA Kd around 10-8 M very high affinity and specificity

Very low affinity or no crossreactivity with close competitors:

Competitors Affinity compared to L-DOPA-BSA
Alpha-methyl-L-DOPA-BSA 2200 times lower low affinity
3-O-methyl-L-DOPA-BSA 50 000 times lower no crossreactivity
Dopamine-BSA 50 000 times lower no crossreactivity
Noradrenaline-BSA 50 000 times lower no crossreactivity
Tyrosine-BSA 50 000 times lower no crossreactivity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Host species

Rabbit

Clonality

Polyclonal

Sub-categories

Catecholamines

Isotype

IgG

Target

Conjugated L-DOPA

Immunogen

Synthetic L-DOPA conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule

Purity

Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation

Form

Lyophilized

Stabilizers / preservatives

None

Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry, Neurobiology, Pharmacology, Thrombosis research

Applications

ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Gemacbio sells as well the corresponding antigen: L-DOPA conjugate (Product number: AG004)

Related antibodies

Gemacbio sells as well antibodies against small molecules of the Phenylalanine pathway: anti-Dopamine (AP001), anti-Adrenaline (AP005), anti-Noradrenaline (AP006), anti-Tyrosine (AP010), anti-Phenylalanine (AP039). Gemacbio sells as well antibodies against other catecholamines: anti-Octopamine (AP007), anti-Tyramine (AP008)