AP001 – Dopamine antibody

Polyclonal anti-conjugated Dopamine antibody (rabbit)

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Additional information

Specifity

Using Dopamine-BSA conjugate (DA-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

Dopamine-BSA Kd around 10-8M very high affinity and specificity

Very low affinity with close competitors:

Competitors Affinity compared to DA-BSA
Noradrenaline-BSA 200 times lower low affinity
L-DOPA-BSA 400 times lower low affinity
Octopamine-BSA 400 times lower low affinity
Tyramine-BSA 5000 times lower very low affinity
Adrenaline-BSA 5000 times lower very low affinity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation)

Additional Information

Host species

Rabbit

Clonality

Polyclonal

Sub-categories

Catecholamines

Isotype

IgG1

Target

Conjugated Dopamine

Immunogen

Synthetic Dopamine conjugated to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule

Purity

Polyclonal antibody was preabsorbed on protein carriers and purified by ammonium sulfate precipitation

Form

Lyophilized

Stabilizers / preservatives

None

Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry, Neurobiology, Pharmacology

Applications

ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Gemacbio sells as well the corresponding antigen: Dopamine conjugate (Product number: AG001)

Related antibodies

Gemacbio also sells the monoclonal anti-Dopamine antibodies (AM001). Gemacbio sells as well antibodies against small molecules of the Phenylalanine pathway: anti-L-DOPA (AP004), anti-Adrenaline (AP005), anti-Noradrenaline (AP006), anti-Octopamine (AP007), anti-Tyramine (AP008), anti-Tyrosine (AP010), anti-Phenylalanine (AP039). Gemacbio sells as well antibodies against other neurotransmitters: anti-Serotonin (AP014), anti-GABA (AP027), anti-Glycine (AP028), anti-L-Glutamate (AP029), anti-Acetylcholine (AP049 or AM002), anti-Histamine (AP032 or AM005)