AM002 – Acetylcholine antibody

Monoclonal anti-conjugated Acetylcholine-like antibody (mouse)

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Specifity

Using Acetylcholine-like-BSA conjugate (ACh-BSA), antibody specificity was performed by using an ELISA test by competition experiments:

Acetylcholine-like-BSA Kd around 10-8 M very high affinity and specificity

No crossreactivity with close competitors:

Competitors Affinity compared to ACh-BSA
Farnesol-BSA 50 000 times lower no crossreactivity
Retinoic acid-BSA 50 000 times lower no crossreactivity
Myristic acid-BSA 50 000 times lower no crossreactivity
Acetylcholine free 50 000 times lower no crossreactivity
Choline free 50 000 times lower no crossreactivity

The antibody recognizes the conjugated form of the small molecule, not the free form (see protocol for specific tissue preparation).

Additional Information

Description

Monoclonal antibody was obtained after ARK mouse immunisation with the conjugate: Choline-bovine serum albumin (BSA). Hybridoma clone was obtained by hybridization of spleen cells with the myeloma cell line SP2/O/Ag14

Host species

Mouse

Clonality

Monoclonal

Clone number

HYChol 001

Sub-categories

Other Neurotransmitters

Isotype

IgG2a, Kappa

Target

Conjugated Choline (Acetylcholine-like)

Immunogen

Synthetic Choline conjugated (Acetylcholine-like) to bovine serum albumin (BSA)

Species reactivity

The antibody recognizes the target in all species, vertebrates (included humans) and invertebrates, because of the universality of the small molecule

Purity

Monoclonal antibody was preadsorbed on protein carriers and purified by ammonium sulfate precipitation

Form

Lyophilized

Stabilizers / preservatives

None

Storage instructions and buffer

Lyophilized vial must be stored at 4°C in a dry area up to 2 years after receipt. After reconstitution with 50% of distilled water and 50% of glycerol, the aliquot must be stored at -20°C, and is stable at least for 2 years. Avoid freeze-thaw cycles

Research areas

Biochemistry, Neurobiology, Neurodegenerative diseases, Pharmacology

Applications

ELISA, Immunohistochemistry (Glutaraldehyde and PFA fixation, see protocol), Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application. Suggested starting dilutions: 1/2000 – 1/10000 for ELISA, 1/1000 – 1/5000 for IHC

Corresponding antigen/related product

To validate the specificity of the response in your biological samples, Gemacbio sells as well the corresponding antigen: Choline (Acetylcholine-like) conjugate (Product number: AG049)

Related antibodies

Gemacbio also sells the polyclonal anti-Acetylcholine-like antibodies (Product number: AP049). Gemacbio sells as well antibodies against other neurotransmitters: anti-Dopamine (AP001 or AM001), anti-Adrenaline (AP005), anti-Noradrenaline (AP006), anti-Serotonin (AP014), anti-GABA (AP027), anti-Glycine (AP028), anti-L-Glutamate (AP029 or AM003), anti-Histamine (AP032 or AM005)