anti-conjugated Tyramine antibodies
Product Number:
AP008
Host:
Rabbit
Clonality:
Polyclonal
Target :
Conjugated Tyramine
Immunogens :
Synthetic tyramine conjugated to protein carriers (PC)
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and ammonium sulphate purified
Form :
Lyophilized
Tested applications :
Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application
Research areas :
Neurobiology, Pharmacology
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate tyramine-PC, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Tyramine-G-PC | 1 |
| Octopamine-G-PC | 1/800 |
| Dopamine-G-PC | 1/>50,000 |
| L.Tyrosine-G-PC | 1/>50,000 |
(a) : Tyramine-G-(Pc) concentration / other conjugated catecholamines concentration at half displacement; G = Glutaraldehyde
Corresponding antigen :
Gemacbio sells the corresponding antigen : Tyramine(BSA) conjugate (code number: AG008)
References :
• GEFFARD M., SEGUELA P. and HEINRICH-ROCK A. M. Antisera against catecholamines : specificity studies and physicochemical data for anti-dopamine and anti-p-tyramine antibodies. Molecular Immunology (1984) 21, 515-522.
• CRISP KM, KLUKAS KA, GILCHRIST LS, NARTEY AJ, Karen A MESCE KA Distribution and development of dopamine and octopamine-synthesizing neurons in the medicinal leech. The Journal of comparative neurology (2002) 442, 115-29
• BLUMENTHAL EM. Regulation of chloride permeability by endogenously produced tyramine in the Drosophila Malpighian tubule. Am J Physiol Cell Physiol. (2003) 284(3), 718-28.
• KITAHAMA K., ARANEDA S., GEFFARD M., SEI C., OKAMURA H. Tyramine-immunoreactive neuronal structures in the rat brain : abundance in the median eminence of the mediobasal hypothalamus. Neuroscience letters (2005) 383(3), 215-9.
• DA SILVA R. and LANGE A.B. Tyramine as a possible neurotransmitter/neuromodulator at the spermatheca of the African migratory locust, Locusta migratoria. Journal of Insect Physiology (2008) 54, 1306-1313.
• KONONENKO N.L., WOLFENBERG H. and PFLUGER H.J. Tyramine as an independent transmitter and a precursor of octopamine in the Locust central nervous system : an immunocytochemical study. The Journal of Comparative Neurology (2009) 512, 433-452.
• BUSCH S., SELCHO M., ITO K. and TANIMOTO H. A map of octopaminergic neurons in the Drosophila brain. The Journal of Comparative Neurology (2009) 513, 643-667.
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com
