anti-conjugated Tryptamine antibodies
Product Number:
AP020
Host:
Rabbit
Clonality:
Polyclonal
Target :
Conjugated tryptamine
Immunogens :
Synthetic tryptamine conjugated to protein carriers (Pc)
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and ammonium sulphate purified
Form :
Lyophilized
Tested applications :
Immunocytochemistry, Immunohistochemistry. Optimal dilutions should be determined by each laboratory for each application
Research areas :
Neurobiology, Pharmacology, Biochemistry, Migraine, Degenerative diseases
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate tryptamine-(Pc), antibody specificity was performed with an ELISA test by competition
experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Tryptamine-G-(Pc) | 1 |
| 5-Hydroxytryptamine-G-(Pc) | 1/50 |
| 5-Methoxytryptamine-G-(Pc) | 1/60 |
| free Tryptamine | 1/2,000 |
| Tryptophan-G-(Pc) | 1/3,000 |
| 5-Methoxytryptophan-G-(Pc) | 1/6,000 |
| 5-Hydroxytryptophan-G-(Pc) | 1/50,000 |
(a) : Tryptamine-G-(Pc) concentration/other conjugated indolealkylamine concentration at half displacement ; G = Glutaraldehyde
Corresponding antigen :
Gemacbio sells the corresponding antigen : Tryptamine(BSA) conjugate (code number : AG020)
References :
• GEFFARD M., TUFFET S., PEUBLE L. and PATEL S. Production of antisera to serotonin and their metabolites and their
use in immunocytochemistry. In Neuronal serotonin (Eds N. N. Osborne and M.Hamon).John Wiley and Sons Ltd., (1988),
pp 1-23.
• TISON F., GEFFARD M. and HENRY P. Tryptamine is found closely associated to the serotoninergic pathways when
using an immunohistochemical method of detection in the rat central nervous system. Biogenic amines, (1990) 7, 235-248.
• DABADIE H., GEFFARD M and PACCALIN J. Simultaneous detection of tryptamine and dopamine in rat substantia nigra and raphe nuclei using specific antibodies. Brain Res., (1990) 512, 138-142.
• DABADIE H. and GEFFARD M., Identification of tryptamine and tryptamine-serotonin neurons in the rat dorsal raphe
nuclei using specific antibodies. Synapse, (1993) 14, 178-183.
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com
