anti-conjugated Taurine antibodies
Product Number:
AP042
Host:
Rabbit
Clonality:
Polyclonal
Target :
Conjugated Taurine
Immunogens :
Synthetic Taurine conjugated to protein carriers (PC)
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and purified
Form :
Lyophilized
Tested applications :
Immunocytochemistry, Immunohistochemistry. Optimal dilutions should be determined by each laboratory for each application
Research areas :
Neurobiology
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate Taurine-Glutaraldehyde-PC, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Taurine-G-PC | 1 |
| Hypotaurine-G-PC | 1/15 |
| GABA-G-PC | 1/500 |
| beta-Alanine-G-PC | 1/1,800 |
| Aspartate-G-PC | 1/5,000 |
| Glycine-G-PC | 1/14,000 |
| Cysteine-G-PC | 1/> 20,000 |
| Glutamate-G-PC | 1/> 20,000 |
| Methionine-G-PC | 1/> 20,000 |
| Free Taurine | 1/> 20,000 |
(a) : Taurine-G-PC concentration/unconjugated or conjugated aminoacids concentration at half displacement.
G = Glutaraldehyde.
Corresponding antigen :
Gemacbio sells the corresponding antigen : Taurine(BSA) conjugate (code number : AG042)
References :
REFERENCES :
• CAMPISTRON G., GEFFARD M. and BUIJS R.M. Immunological approach to the detection of taurine and immunocytochemical results. J. Neurochem. (1986) 46, 862-868.
• BUSTAMANTE J, LOBO MV, ALONSO FJ, MUKALA NT, GINÉ E, SOLÍS JM, TAMARIT-RODRIGUEZ J, MARTÍN DEL RÍO R. An osmotic-sensitive taurine pool is localized in rat pancreatic islet cells containing glucagon and somatostatin. Am J Physiol Endocrinol Metab. (2001) 281(6), 1275-85.
• LOBO MV, ALONSO FJ, LATORRE A, DEL RÍO RM. Immunohistochemical localization of taurine in the rat ovary, oviduct, and uterus. J Histochem Cytochem. (2001) 49(9), 1133-42.
• LOBO MV, ALONSO FJ, DEL RÍO RM. Immunohistochemical localization of taurine in the male reproductive organs of the rat. J Histochem Cytochem. (2000) 48(3), 313-20.
• SINAKEVITCH I., FARRIS SM.and STRAUSFELD NJ. Taurine-, aspartate-, glutamate-like immunoreactivity identifies chemically distinct subdivisions of kenyon cells in the cockroach mushroom body. J. Comp. Neurol. (2001) 439(3), 352-67.
• SJÖHOLM M, SINAKEVITCH I, STRAUSFELD NJ, IGNELL R and HANSSON BS. Functional division of intrinsic neurons in the mushroom bodies of male Spodoptera littoralis revealed by antibodies against aspartate, taurine, FMRF-amide, Mas-allatotropin and DC0. Arthropod Struct Dev. (2006) 35(3), 153-68.
• SINAKEVITCH I, SJÖHOLM M, HANSSON BS and STRAUSFELD NJ. Global and local modulatory supply to the mushroom bodies of the moth Spodoptera littoralis. Arthropod Struct Dev. (2008) 37(4), 260-72.
• STRAUSFELD NJ, SINAKEVITCH I, BROWN SM and FARRIS SM Ground plan of the insect mushroom body : functional and evolutionary implications. J. Comp. Neurol. (2009) 513, 265-91.
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com
