anti-conjugated Serotonin (5-dihydroxytryptamine) antibodies
Target :
Conjugated 5-Hydroxytryptamine
Immunogens :
Synthetic 5-Hydroxytryptamine conjugated to protein carriers (Pc)
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and purified
Form :
Lyophilized
Tested applications :
Immunocytochemistry, Immunohistochemistry. Optimal dilutions should be determined by each laboratory for each application
Research areas :
Neurobiology, Biochemistry, Pharmacology, Neurodegenerative diseases
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate 5-Hydroxytryptamine-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Serotonin-G-(Pc) | 1 |
| 5-Methoxytryptamine-G-(Pc) | 1/25 |
| Tryptamine-G-(Pc) | 1/50 |
| 5-Hydroxytryptophan-G-(Pc) | 1/175 |
| L-Tryptophan-G-(Pc) | 1/3,500 |
| 5-Methoxytryptophan-G-(Pc) | 1/20,000 |
| free Serotonin | 1/1,15 |
(a) : 5-Hydroxytryptamine-G-(Pc) concentration / Other c conjugated indolealkylamines concentration at half displacement ; G = Glutaraldehyde
Corresponding antigen :
Gemacbio sells the corresponding antigen : 5-Hydroxytryptamine conjugate (code number : AG014)
References :
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