anti-conjugated Octopamine antibodies
Product Number:
AP007
Host:
Rabbit
Clonality:
Polyclonal
Target :
Conjugated octopamine
Immunogens :
Synthetic octopamine conjugated to protein carrier (PC)
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and ammonium sulphate purified
Form :
Lyophilized
Tested applications :
Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application
Research areas :
Neurobiology, Pharmacology
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate octopamine-Pc, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Octopamine-G-PC | 1 |
| Tyramine-G-PC | 1/400 |
| Noradrenaline-G-PC | 1/10,000 |
| Tyrosine-G-PC | 1/>50,000 |
| Dopamine-G-PC | 1/100,000 |
(a) : Octopamine-G-(Pc) concentration / other conjugated catecholamine concentration at half displacement; G = Glutaraldehyde
Corresponding antigen :
Gemacbio sells the corresponding antigen: Octopamine conjugate (code number: AG007)
References :
• MONS N. and GEFFARD M. Specific antisera against the catecholamines : L-3,4- dihydroxyphenylalanine, dopamine, noradrenaline and octopamine tested by and enzyme-linked immunosorbent assay. J. Neurochem., (1987) 48, 1826-1833.
• SINAKEVITCH I., GEFFARD M., PELHATE M. and LAPIED B. Octopamine-like immunoreactivity in the dorsal unparied median (DUM) neurons innervating the accessory gland of the male cockroach Periplaneta americana. Cell and Tissue Res., (1994) 276, 15-21.
• SINAKEVITCH I., GEFFARD M., PELHATE M. and LAPIED B. Octopaminergic dorsal unpaired median (DUM) neurones innervating the collaterial glands of the female cockroach Periplaneta americana. The J. of Exp. Biol., (1995) 198, 1539-1544.
• STRAUSFELD N.J., SINAKEVITCH I., and VILINSKY I. The mushroom bodies of Drosophila melanogaster : an immunocytological and golgi study of Kenyon cell organization in the calyces and lobes. Microscopy research and technique (2003) 62, 151-169.
• WIDMER A, HOGER U, MEISNER S, FRENCH AS and TORKKELI PH Spider Peripheral Mechanosensory Neurons Are Directly Innervated and Modulated by Octopaminergic Efferents. The Journal of Neuroscience (2005) 25(6), 1588-1598.
• BLEEKER A. K. ; VAN DER ZEE B ; SMID H.M. Octopamine-like immunoreactivity in the brain and suboesophageal ganglion of two parasitic wasps, Cotesia glomerata and Cotesia rubecula. Animal biology (2006) 56(2), 247-257.
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com
