anti-conjugated Noradrenaline antibodies
Product Number:
AP006
Host:
Rabbit
Clonality:
Polyclonal
Target :
Conjugated noradrenaline
Immunogens :
Synthetic noradrenaline conjugated to protein carriers (PC)
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and ammonium sulphate purified
Form :
Lyophilized
Tested applications :
Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application
Research areas :
Neurobiology, Pharmacology, Cardiovascular research, Neurodegenerative diseases
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate noradrenaline-PC, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Noradrenaline-G-PC | 1 |
| Octopamine-G-PC | 1/30 |
| Dopamine-G-PC | 1/70 |
| Adrenaline-G-PC | 1/180 |
| L-DOPA-G-PC | 1/>5,000 |
| Tyramine-G-PC | 1/>5,000 |
| Noradrenaline | 1/>5,000 |
(a) : Noradrenaline-G-PC concentration / other conjugated catecholamines concentration at half displacement ; G = Glutaraldehyde
Corresponding antigen :
Gemacbio sells the corresponding antigen : Noradrenaline conjugate (code number : AG006)
References :
• FRITSCHY J.M., GEFFARD M. and GRZANNA R. The response of noradrenergic axons to systematically administered DSP-4 in the rat : an immunohistochemical study using antibodies to noradrenaline and dopamine ß-hydroxylase. J. of Chemical Neuroanatomy (1990) 3 (4), 309-321.
• RAJAOFETRA N., POULAT N., MARLIER L., SANDILLON F., DRIAN M.J., KONIG N., FAMOSE F., VERSCHUERE B., GOUY D., GEFFARD M. and PRIVAT A. Transplantation of embryonic serotonin immunoreactive neurons into the transected spinal cord of adult monkey (Macaca fascicularis). Brain Res. (1992) 572, 329-334.
• SINGH S., JOHNSON P.I., JAVED A.BS., GRAY T.S., LONCHYNA V.A. and WURSTER R.D. Monoamine- and histamine-synthesizing enzymes and neurotransmitters within neurons of adult human cardiac ganglia. Circulation (1999) 99(3), 411-419.
• HERNESS S., ZHAO F.L., KAYA N., LU S.G., SHEN T. and SUN X.D. Adrenergic signalling between rat taste receptor cells. Journal of Physiology (2002) 543(2), 601-614.
• SELVAGE D.J., LEE S.Y., PARSONS L.H., SEO D.O. and RIVIER C.L. A hypothalamic-testicular neural pathway is influenced by brain catecholamines, but not testicular blood flow. Endocrinology (2004) 145, 1750-9.
• COLL M, GENESCÀ J, RAURELL I, RODRÍGUEZ-VILARRUPLA A, MEJÍAS M, OTERO T, ORIA M, ESTEBAN R, GUARDIA J, BOSCH J, MARTELL M. Down-regulation of genes related to the adrenergic system may contribute to splanchnic vasodilation in rat portal hypertension. J Hepatol. (2008) 49(1), 43-51.
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