anti-conjugated Nitroso-L.Glutathione antibodies
Product Number:
AP073
Host:
Rat
Clonality:
Polyclonal
Target :
Conjugated Nitroso-L.Glutathione
Immunogens :
Synthetic Nitroso-L.Glutathione conjugated to protein carriers (PC)
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and purified
Form :
Lyophilized
Tested applications :
Immunocytochemistry, Immunofluorescence. Optimal dilutions should be determined by each laboratory for each application.
Research areas :
Neurobiology, Immunology, Pharmacology, Degenerative diseases, Plant Physiology
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate Nitroso-L.Glutathione-Glutaraldehyde-PC, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
| Compounds | Cross-reactivity ratio(a) |
|---|---|
| Nitroso-L.Glutathione-G-PC | 1 |
| L.Glutathione-G-PC | 1/20 |
| Nitroso-L.Cysteine-G-PC | 1/600 |
| Nitroso-L.Tryptophan-G-PC | 1/500 |
| Nitroso-L.Tyrosine-G-PC | 1/1,000 |
| Glycine-G-PC | 1/12,000 |
| L.Glutamate-G-PC | 1/26,000 |
(a) : Nitroso-L.Glutathione-G-PC concentration/conjugated close-related compounds concentration at half displacement.
G = Glutaraldehyde
Corresponding antigen :
Gemacbio sells the corresponding antigen : Nitroso-L.Glutathione conjugate (code number: AG073)
References :
• BARROSO JB, CORPAS FJ, CARRERAS A, RODRIGUEZ-SERRANO M, ESTEBAN FJ, FERNANDEZ-OCANA A, CHAKI M, ROMERO-PUERTAS MC, VALDERRAMA R, SANDALIO LM, DEL RIO LA. Localization of S-nitrosoglutathione and expression of S-nitrosoglutathione reductase in pea plants under cadmium stress. J Exp Bot. (2006) 57(8):1785-93.
• VALDERRAMA R, CORPAS FJ, CARRERAS A, FERNANDEZ-OCANA A, CHAKI M, LUQUE F, GOMEZ-RODRIGUEZ MV, COLMENERO-VAREA P, DEL RIO LA, BARROSO JB. Nitrosative stress in plants. FEBS Lett. (2007) 581(3):453-61.
• CORPAS FJ, CARRERAS A, ESTEBAN FJ, CHAKI M, VALDERRAMA R, DEL RIO LA, BARROSO JB. Localization of S-nitrosothiols and assay of nitric oxide synthase and S-nitrosoglutathione reductase activity in plants. Methods Enzymol. (2008) 437:561-74.
• CHAKI M, FERNÁNDEZ-OCAÑA AM, VALDERRAMA R, CARRERAS A, ESTEBAN FJ, LUQUE F, GÓMEZ-RODRÍGUEZ MV, BEGARA-MORALES JC, CORPAS FJ, BARROSO JB.Involvement of reactive nitrogen and oxygen species (RNS and ROS) in sunflower-mildew interaction. Plant Cell Physiol. (2009) 50(2):265-79.
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