anti-conjugated Nitroso-L.Cysteine antibody

Target :

Conjugated Nitroso-L-Cysteine

Immunogens :

Synthetic Nitroso-L-Cysteine conjugated to protein carrier (Pc)

Quantity :
100µl
 

Isotype :
IgG

Clone number :
HY8E12

Purity :
previously preabsobed on protein carriers, and purified

Form :
Lyophilized

Tested applications :
Immunocytochemistry, Immunohistochemistry, Immunoblotting. Optimal dilutions should be determined by each laboratory for each application

Research areas :

Neurobiology, Immunology, Pharmacology, Degenerative diseases

Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.

Specificity :

Using a conjugate Nitroso-L-Cysteine-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :

(a) : NO-L-Cysteine-G-(Pc) concentration / amino-acid concentration at half displacement ; G = Glutaraldehyde
 

Corresponding antigen :

Gemacbio sells the corresponding antigen : Nitroso-L-Cysteine conjugate (code number : AG122)

References :

• MNAIMNEH S, GEFFARD M, VEYRET B, VINCENDEAU P. Albumin nitrosylated by actived macrophages possesses anti-parasitic effects neutralized by NO-acetylated-cysteine antibodies. J. of Immunology, (1997), 158, 308-314.
• MNAIMNEH S, GEFFARD M, VEYRET B, VINCENDEAU P. Detection of nitrosylated epitopes in Trypanosoma brucei gambiense by polyclonal and monoclonal anti-conjugated-NO-cysteine antibodies. CR Acad Sci III. 1999, 322(4):311-22.
• LORCH S.A., FOUST R., GOW A., ARKOVITZ M., SALZMAN A.L., SZABO C., VEYRET B., GEFFARD M. and ISCHIROPOULOS H. Immunohistochemical localization of protein 3-nitrotyrosine and S-nitrosocysteine in a murine model of inhaled nitric oxide therapy. Pediatric research (2000) 47(6), 798-805.
• ALENCAR J.L., LOBYSHEVA I., GEFFARD M., SARR M., SCHOTT C., SCHINI-KERTH V., NEPVEU F., STOCLET J.C. and MULLER B. Role of S-nitrosation of cysteine residues in long-lasting inhibitory effect of nitric oxide on arterial tone. Molecular Pharmacology (2003) 63(5), 1148-1158.
• ALENCAR J.L., LOBYSHEVA I., CHALUPSKY K., GEFFARD M., NEPVEU F., STOCLET J.C. and MULLER B. S-nitrosating nitric oxide donors induce long-lasting inhibition of contraction in isolated arteries. J. Pharmacol. Exp. Ther. (2003) 307(1), 152-159.
• MNAIMNEH S., DAMAJ M., BARHOUMI R., MOUNEIMNE Y., GEFFARD M., VEYRET B. and VINCENDEAU P. Evidence for nitric oxide involvement in experimental autoimmune encephalomyelitis and adjuvant-induced arthritis in Lewis rat. The pain clinic (2004) 16(3), 229-243.
• GRECO TM, HODARA R, PARASTATIDIS I, HEIJNEN HF, DENNEHY MK, LIEBLER DC, ISCHIROPOULOS H. Identification of S-nitrosylation motifs by site-specific mapping of the S-nitrosocysteine proteome in human vascular smooth muscle cells. Proc Natl Acad Sci U S A. 2006 May 9;103(19):7420-5.
• SHI Q, CHEN HF, LOU YJ. Further evidence that rat liver microsomal glutathione transferase 1 is not a cellular protein target for S-nitrosylation. Chem Biol Interact. 2006 Sep 25;162(3):228-36.
• KADEKARO M, SU G, CHU R, LEI Y, LI J, FANG L. Effects of nitric oxide on expressions of nitrosocysteine and calcium-activated potassium channels in the supraoptic nuclei and neural lobe of dehydrated rats. Neurosci Lett. 2007 Jan 10;411(2):117-22.
• ZUCKERBRAUN BS, STOYANOVSKY DA, SENGUPTA R, SHAPIRO RA, OZANICH BA, RAO J, BARBATO JE, TZENG E. Nitric oxide-induced inhibition of smooth muscle cell proliferation involves S-nitrosation and inactivation of RhoA. Am J Physiol Cell Physiol. 2007 Feb;292(2):C824-31.
• SELEMIDIS S, DUSTING GJ, PESHAVARIYA H, KEMP-HARPER BK, DRUMMOND GR. Nitric oxide suppresses NADPH oxidase-dependent superoxide production by S-nitrosylation in human endothelial cells. Cardiovasc Res. 2007 Jul 15;75(2):349-58.

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