anti-conjugated Nitroso-L.Cysteine antibodies

Target :

Conjugated Nitroso-L-Cysteine

Immunogens :

Synthetic Nitroso-L-Cysteine conjugated to protein carrier (Pc)

Quantity :
100µl
 

Isotype :
IgG

Purity :
previously preabsobed on protein carriers, and purified

Form :
Lyophilized

Tested applications :
Immunohistochemistry. Optimal dilutions should be determined by each laboratory for each application

Research areas :

Neurobiology, Immunology, Pharmacology, Degenerative diseases

Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.

Specificity :

Using a conjugate Nitroso-L-Cysteine-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :

(a) : NO-L-Cysteine-G-(Pc) concentration / amino-acid concentration at half displacement ; G = Glutaraldehyde
 

Corresponding antigen :

Gemacbio sells the corresponding antigen : Nitroso-L-Cysteine conjugate (code number : AG122)

References :

• GRECO TM, HODARA R, PARASTATIDIS I, HEIJNEN HF, DENNEHY MK, LIEBLER DC, ISCHIROPOULOS H. Identification of S-nitrosylation motifs by site-specific mapping of the S-nitrosocysteine proteome in human vascular smooth muscle cells. PNAS (2006) 103(19):7420-5.
• CHRISTEN S, CATTIN I, KNIGHT I, WINYARD PG, BLUM JW, ELSASSER TH. Plasma S-nitrosothiol status in neonatal calves: ontogenetic associations with tissue-specific S-nitrosylation and nitric oxide synthase. Exp Biol Med (Maywood) (2007) 232(2):309-22.

For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com

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