anti-conjugated Nitro-L.Tyrosine antibodies
Product Number:
AP065
Host:
Rabbit
Clonality:
Polyclonal
Target :
Conjugated NO2-L.Tyrosine
Immunogens :
Synthetic NO2-L.Tyrosine conjugated to protein carrier (PC)
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and purified
Form :
Lyophilized
Applications notes :
Optimal dilutions should be determined by each laboratory for each application
Research areas :
Neurobiology, Immunology, Pharmacology, Degenerative diseases
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate NO2-L.Tyrosine-Glutaraldehyde-(PC), antibody specificity was performed with an ELISA test by competition experiments with the following compounds:
| Compounds | Cross-reactivity ratio(a) |
|---|---|
| Nitro-L.Tyrosine-G-PC | 1 |
| Nitroso-L.Tyrosine-G-PC | 1/250 |
| L.Tyrosine-G-PC | 1/> 100,000 |
| Nitro-Serotonin-G-PC | 1/> 100,000 |
| Nitroso-Serotonin-G-PC | 1/> 100,000 |
| Nitroso-L.Cysteine-G-PC | 1/> 100,000 |
| Nitroso-LTryptophan-G-PC | 1/> 100,000 |
| Nitroso-L.Histidine-G-PC | 1/> 100,000 |
(a) : NO2-L.Tyrosine-G-PC concentration/conjugated close-related compounds concentration at half displacement.
G = Glutaraldehyde, PC = Protein Carrier.
Corresponding antigen :
Gemacbio sells the corresponding antigen : Nitro-L.Tyrosine(BSA) conjugate (code number : AG065)
References :
Banerjee A., Zhang X., Reddy Manda K., Banks WA. and Ercal N. HIV proteins (gp120 and Tat) and methamphetamine in oxidative stress-induced damage in the brain: Potential role of the thiol antioxidant N-acetylcysteine amide. Free Radical Biology and Medicine (2010) 48(10), 1388-98.
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com
