anti-conjugated Nicotinamide (Vitamin B3 or PP) antibodies
Product Number:
AP121
Host:
Rat
Clonality:
Polyclonal
Target :
Conjugated Nicotinamide
Immunogens :
Synthetic Nicotinamide conjugated to proteins carriers
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and ammonium sulphate purified
Form :
Lyophilized
Tested application :
Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application.
Research areas :
Biochemistry (metabolic ways)
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate Nicotinamide-protein carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Nicotinamide-BSA | 1 |
| Pyridoxine-BSA | 1/30 |
| Pyridoxal-BSA | 1/9,000 |
| Pyridoxal (reduced)-BSA | 1/10,000 |
(a) : Nicotinamide-BSA concentration/conjugated compound concentration at half displacement.
BSA = Bovine Serum Albumin
Corresponding antigen :
Gemacbio sells the corresponding antigen : Nicotinamide (BSA) conjugate (code number : AG121)
Reference :
MANGAS A, COVENAS R, BODET D, DULEU S, MARCOS P, GEFFARD M Vitamins in the monkey brain : an immunocytochemical study. J. Chem. Neuroanat. (2009) 38(1), 1-8
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com
