anti-conjugated L.DOPA antibodies

Target :

Conjugated L-dihydroxyphenylalanine (L-DOPA)

Immunogens :

Synthetic L-DOPA conjugated to protein carriers (Pc)

Quantity :
100µl

Isotype :
IgG

Purity :
previously preabsobed on protein carriers, and purified

Form :
Lyophilized

Tested applications :

Immunocytochemistry, Immunohistochemistry. Optimal dilutions should be determined by each laboratory for each application

Research areas :

Neurobiology, Thrombosis research

Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.

Specificity :

Using a conjugate L-DOPA-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :

  (a) : L-DOPA-G-(Pc) concentration /other conjugated catecholamine concentration at half displacement ; G = Glutaraldehyde

Corresponding antigen :

Gemacbio sells the corresponding antigen: L-dihydroxyphenylalanine conjugate (code number : AG004)

References :

• MONS N., DANEL N and GEFFARD M. Visualization of L-dihydroxyphenylalanine in rat brain by using specific antibodies. Brain Res., (1988), 451, 403-407.
• OKAMURA H., KITAHAMA K., MONS. N., IBATA Y., JOUVET M. and GEFFARD M. L-DOPA-immunoreactive neurons in the rat hypothalamic tuberal region. Neurosci. Letters, (1988), 95, 42-46.
• KITAHAMA K., MONS N., OKAMURA M., JOUVET M. and GEFFARD M. Endogenous L-DOPA, its immunoreactivity in neurons of midbrain and its projection fields in the cat. Neurosci. Letters, (1988), 95, 47-52.
• MONS N., TISON F. and GEFFARD M. Identification of L-DOPA-dopamine and L-DOPA cell bodies in the rat mesencephalic dopaminergic cell systems. Synapse, (1989), 4, 99-105.
• TISON F., MONS N., ROUET-KARAMA S., GEFFARD M. and HENRY P. Endogenous L-DOPA in the rat dorsal vagal complex : an immunocytochemical study by light and election microscopy. Brain Res., (1989), 497, 260-270.
• MONS N., TISON F. and GEFFARD M. Existence of L-DOPA immunoreactive neurons in the rat preoptic area and anterior hypothalamus. Neuroendocrinology, (1990) 51, 425-428.
• TISON F., MONS N. , GEFFARD M. and HENRY P. Immunohistochemistry of endogenous L-DOPA in the rat posterior hypothalamus. Histochemistry, (1990) 93, 655-660.
• MANIER M., FEUERSTEIN C., PASSAGIA J.G., MOUCHET P., MONS N., GEFFARD M. and THIBAULT J. Evidence for the existence of L-DOPA-and dopamine-immunoreactive nerve cell bodies in the candal part of the dorsal motor nucleus of the ragus nerve. J. Chem. Neuroanat., (1990) 3, 193-206.
• KITAHAMA K., GEFFARD M., OKAMURA H., NAGATSU I., MONS N., JOUVET M. Dopamine and DOPA-immunoreactive neurons in the cat forebrain with reference to tyrosine hydroxylase-immunohistochemistry. Brain Res., (1990), 518, 83-94.
• OKAMURA H., KITAHAMA K., MONS N., MATSUMOTO Y., IBATA Y. and GEFFARD M. Heterogeneous distribution of L-DOPA immunoreactivity in dopaminergic neurons of the rat midbrain. In "Basic, clinical, an therapeutic aspects of Alzheimer's and Parkinson's diseases" (Eds. NAGATSU T., FISHER A. and YOSHIDA M.) Plenum Press, New-York and London, (1991), 423-426.
• TISON F., MONS N., GEFFARD M. and HENRY P. The metabolism of exogenous L-DOPA in the brain: an immunohistochemical study of its conversion to dopamine in non-catecholaminergic cells of the rat brain. J. of Neural Transmission Park Dis Dement Sect, (1991) 3 (1), 27-39.
• LAGIER B., CHARRIER M.C., GEFFARD M. and DOUTREMEPUICH C. Effects of polyclonal antibodies against conjugated L-DOPA or against conjugated acetylcholine on experimental venous thrombosis. Thrombosis Res., (1992) 65, 275-280.
• ARAI R., KARASAWA N., GEFFARD M., CALAS A., NAGATSU T. and NAGATSU I. Immunohistochemical evidence that central serotonin neurons produce dopamine from exogenous L-DOPA in the rat, with reference to the involvement of aromatiL- amino acid decarboxylase. Brain Res., (1994) 667, 295-299.
• ARAI R., KARASAWA N., GEFFARD M. and NAGATSU I. L-DOPA is converted to dopamine in serotonergic fibers of the stratium of the rat : a double-labeling immunofluorescence study. Neurosc. Letters, (1995) 195, 195-198.
• YUE J.-L., OKAMURA H., GOSHIMA Y., NAKAMURA S., GEFFARD M. and MISU Y. Baroreceptor-aortic nerve-mediated release of endogenous L-3,4-dihydroxyphenylalanine and its tonic depressor function in the nucleus tractus solitarii of rats. Neuroscience, (1994) 62 (1), 145-161.
• NGUYEN-LEGROS J, KRIEGER M, SIMON A. Immunohistochemical localization of L-DOPA and aromatic L-amino acid-decarboxylase in the rat retina. Invest Ophthalmol Vis Sci. 1994 Jun;35(7):2906-15.

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