anti-conjugated L.Aspartic acid (L.Aspartate) antibodies
Target :
Conjugated L-aspartic acid
Immunogens :
Synthetic L-aspartic acid conjugated to protein carriers (Pc)
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and ammonium sulphate purified
Form :
Lyophilized
Tested applications :
Immunocytochemistry, Immunohistochemistry. Optimal dilutions should be determined by each laboratory for each application
Research areas :
Neurobiology, Pharmacology, Degenerative diseases
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate L-aspartic acid-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| L-Aspartic acid-G-(Pc) | 1 |
| L-Glutamic acid-G-(Pc) | 1/30,000 |
| GABA-G-(Pc) | 1/>100,000 |
(a) : L-Aspartic-G-(Pc) concentration / other conjugated aminoacids concentration at half displacement ; G = Glutaraldehyde
Corresponding antigen :
Gemacbio sells the corresponding antigen : L-Aspartatic acid (BSA) conjugate (code number : AG025)
References :
• CAMPISTRON G., BUIJS R.M. and GEFFARD M. Specific antibodies against aspartate and their immunocytochemical application in the rat brain. Brain Res. (1986) 365, 179-184.
• SHERRY D.M., ST-JULES R.S. and TOWNES-ANDERSON E. Morphologic and neurochemical target selectivity of regenerating adult photoreceptors in vitro. J. Comp. Neurol. (1996) 376, 476-488.
• SINAKEVITCH I., FARRIS SM.and STRAUSFELD J. Taurine-, aspartate-, glutamate-like immunoreactivity identifies chemically distinct subdivisions of kenyon cells in the cockroach mushroom body. J. Comp. Neurol.. (2001) 439(3), 352-67.
• SJÖHOLM M, SINAKEVITCH I, STRAUSFELD NJ, IGNELL R, HANSSON BS. Functional division of intrinsic neurons in the mushroom bodies of male Spodoptera littoralis revealed by antibodies against aspartate, taurine, FMRF-amide, Mas-allatotropin and DC0. Arthropod Struct Dev. (2006) 35(3), 153-68.
• STRAUSFELD NJ, SINAKEVITCH I, BROWN SM and FARRIS SM Ground plan of the insect mushroom body : functional and evolutionary implications. J. Comp. Neurol. (2009) 513, 265-91.
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com

