anti-conjugated Homocysteine antibodies
Target :
Conjugated Homocysteine
Immunogens :
Synthetic conjugated Homocysteine to protein carriers
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and ammonium sulphate purified
Form :
Lyophilized
Tested applications :
Dot-Blot. Optimal dilutions should be determined by each laboratory for each application.
Research areas :
Biochemistry, Neurobiology, Radical processes, Degenerative diseases
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate Homocysteine-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Homocysteine-G-BSA | 1 |
| Cysteine-G-BSA | 1/450 |
| Homocysteic acid-G-BSA | 1/> 100,000 |
(a) : Homocysteine-G-BSA concentration/Other conjugated aminoacids concentration at half displacement ;
G = Glutaraldehyde, BSA : Bovine Serum Albumin.
Corresponding antigen :
Gemacbio sells the corresponding antigen: Homocysteine(BSA) conjugate (code number: AG035)
References :
• Van Campenhout A., Moran CS., Parr A., Clancy P., Rush C., Jakubowski H. and Golledge MChir J. Role of homocysteine in aortic calcification and osteogenic cell differentiation. Atherosclerosis (2009) 202(2), 557–566.
• Ganapathy P., Moister B., Roon P., Mysona B., Duplantier JN., Dun Y., Moister TV., Farley MJ., Prasad PD., Liu K. and Smith SB. Endogenous elevation of homocysteine induces retinal neuron death in the cystathionine-β-synthase mutant mouse. Investigative Ophthalmology and Visual Science (2009) 50, 4460-70.
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com

