anti-conjugated Histamine antibody
Product Number:
AM005
Host:
Mouse
Clonality:
Monoclonal
Target :
Conjugated histamine
Immunogens :
Synthetic histamine conjugated to protein carrier (Pc)
Quantity :
100µl
Isotype :
IgA, Kappa
Clone number :
HyHIS1
Purity :
The ascitic fluid was purified by ammonium sulfate precipitation
Form :
Lyophilized
Tested application :
Immunohistochemistry. Optimal dilutions should be determined by each laboratory for each application.
Research areas :
Neurobiology, Immunology
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate histamine-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Histamine-HD-(Pc) | 1 |
| 1-Methyl-Histamine-HD-(Pc) | 1 |
| Histamine-G-(Pc) | 1/> 50,000 |
| Histamine-GA-(Pc) | 1/> 50,000 |
| Histidine-HD-(Pc) | 1/> 50,000 |
(a) : Histamine-HD-(Pc) concentration/ other conjugated amino acids concentration at half displacement ; HD : Hexamethylene diisocyanate ; G : Glutaraldehyde ; GA : Glutaric anhydride
Corresponding antigen :
Gemacbio sells the corresponding antigen: Histamine-HD-BSA conjugate (code number: AG145)
Reference :
GRUETTER CA, DAVIS SC, HAYES JR, CLARK SL and GREEN TL Comparison of histamine content in B16F10 melanoma cells and non-tumorigenic Melan-A melanocytes. Experimental Biology (2009), Poster 740.20.
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com
