anti-conjugated Histamine antibodies
Product Number:
AP032
Host:
Rabbit
Clonality:
Polyclonal
Target :
Conjugated Histamine
Immunogens :
Synthetic Histamine conjugated to protein carriers
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and ammonium sulphate purified
Form :
Lyophilized
Tested applications :
Immunohistochemistry, Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application.
Research areas :
Neuroscience
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate Histamine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Histamine-G-BSA | 1 |
| 1-Methylhistamine-G-BSA | 1/> 50,000 |
| 3-Methylhistamine-G-BSA | 1/> 50,000 |
| L-Histidine-G-BSA | 1/> 50,000 |
(a) : Histamine-G-BSA concentration/Other conjugated aminoacid concentration at half displacement ;
BSA = Bovine Serum Albumin.
Corresponding antigen :
Gemacbio sells the corresponding antigen: Histamine conjugate (code number: AG032)
References :
• MANETA-PEYRET L., MOREAU P., DULLUC J. and GEFFARD M. Antibodies to histamine. Specificity studies and radioimmunological assay. J. Immunol. methods (1986) 90, 39-45.
• SINGH S., JOHNSON P.I., JAVED A.BS., GRAY T.S., LONCHYNA V.A. and WURSTER R.D. Monoamine- and histamine-synthesizing enzymes and neurotransmitters within neurons of adult human cardiac ganglia. Circulation (1999) 99(3), 411-419.
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com
