anti-conjugated Glutathione antibodies

Product Number: 
AP047
Host: 
Rabbit
Clonality: 
Polyclonal
Gemacbio

Target :

Conjugated Glutathione


Immunogens :

Synthetic Glutathione conjugated to bovine serum albumin (BSA)


Quantity :
100µl


Isotype :
IgG


Purity :
previously preabsobed on protein carriers, and purified


Form :
Lyophilized


Tested applications :
Immunohistochemistry, Immunosensor detection. Optimal dilutions should be determined by each laboratory for each application


Research areas :

Neuroscience


Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.


Specificity :

Using a conjugate Glutathione-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :

          Compounds          Cross-reactivity ratio (a)
Glutathione-G-BSA 1
free Glutathione 1/>50,000
Cysteine-G-BSA 1/>50,000
Glutamate-G-BSA 1/>50,00

(a) : Glutathione-G-BSA concentration /unconjugated or conjugated analogs concentration at half displacement.
 G = Glutaraldehyde, BSA = Bovine Serum Albumin.

Corresponding antigen :

Gemacbio sells the corresponding antigen : Glutathione conjugate (code number : AG047)


References :

• GERDON AE, WRIGHT D.W. and CLIFFEL D.E. Quartz crystal microbalance detection of glutathione-protected
nanoclusters using antibody recognition. Anal. Chem. (2005) 77, 304-310.
• KOLB D., MULLER M., ZELLNIG G. and ZECHMANN B. Cadmium induced changes in subcellular glutathione contents
within glandular trichomes of Cucurbita pepo L. Protoplasma (2009).
• ZECHMANN B. and MULLER M. Subcellular compartmentation of glutathione in dicotyledonous plants. Protoplasma
(2010).
• ZECHMANN B., TOMASIC A., HORVAT L. and FULGOSI H. Subcellular distribution of glutathione and cysteine in
cyanobacteria. Protoplasma (2010).

 

For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com


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