anti-conjugated D.Serine antibodies
Product Number:
AP041
Host:
Rabbit
Clonality:
Polyclonal
Target :
Conjugate D-Serine
Immunogens :
Synthetic D-Serine conjugated to proteins carriers
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and ammonium sulphate purified
Form :
Lyophilized
Tested applications :
Immunohistochemistry, Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application.
Research areas :
Neuroscience (neuromodulators), Biochemistry (stereochemistry studies).
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate D-Serine-Glutaraldehyde-protein carrier (BSA), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| D-Serine-G-BSA | 1 |
| L-Serine-G-BSA | 1/> 50,000 |
| D.Threonine-G-BSA | 1/> 50,000 |
| L.Threonine-G-BSA | 1/> 50,000 |
| D-Cysteine-G-BSA | 1/> 50,000 |
| D-Alanine-G-BSA | 1/> 50,000 |
| L.Glutamate-G-BSA | 1/> 50,000 |
| L.Glutamine-G-BSA | 1/> 50,000 |
| L.Aspartate-G-BSA | 1/> 50,000 |
| D.Aspartate-G-BSA | 1/> 50,000 |
| GABA-G-BSA | 1/> 50,000 |
| Glycine-G-BSA | 1/> 50,000 |
| Taurine-G-BSA | 1/> 50,000 |
(a) : D-Serine-G-BSA concentration/other conjugated aminoacids concentration at half displacement ;
G = Glutaraldehyde.
BSA = Bovine Serum Albumin
Corresponding antigen :
Gemacbio sells the corresponding antigen : D-Serine conjugate (code number : AG041)
References :
• MOTHET JP., POLLEGIONI L., OUANOUNOU G., MARTINEAU M., FOSSIER P. and BAUX G. Glutamate receptor activation triggers a calcium-dependent and SNARE protein-dependent release of the gliotransmitter D-serine. PNAS (2005) 102(15), 5606-11.
• DEMEMES D. MOTHET JP. and NICOLAS M-T. Cellular distribution of D-Serine, Serine racemase and D-Amino acid oxidase in the rat vestibular sensory epithelia. Neuroscience (2006) 137(3), 991-997.
• KARTVELISHVILY E, SHLEPER M, BALAN L, DUMIN E and WOLOSKER H. Neuron-derived D-serine release provides a novel means to activate N-methyl-D-aspartate receptors. J Biol Chem. (2006) 281(20), 14151-62.
• MARTINEAU M, GALLI T, BAUX G, MOTHET JP. Confocal imaging and tracking of the exocytotic routes for D-serine-mediated gliotransmission. Glia (2008) 56(12), 1271-84.
• LIU YH, WANG L, WEI LC, HUANG YG, CHEN LW Up-regulation of D-serine might induce GABAergic neuronal degeneration in the cerebral cortex and hippocampus in the mouse pilocarpine model of epilepsy. Neurochem Res. (2009) 34(7), 1209-18.
• KALBAUGH TL, ZHANG J, DIAMOND JS. Coagonist release modulates NMDA receptor subtype contributions at synaptic inputs to retinal ganglion cells. J Neurosci. (2009) 29(5), 1469-79.
• VILLAR-CERVINO V., BARREIRO-IGLESIAS A., RODICIO MC. And ANADON R. D-Serine is distributed in neurons in the brain of the sea lamprey. The Journal of Comparative Neurology (2010) 518, 1688-1710.
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