anti-conjugated Dopamine antiboides

Target :

Conjugated Dopamine

Immunogens :

Synthetic Dopamine conjugated to protein carriers (Pc)

Quantity :
100µl

Isotype :
IgG

Purity :
previously preabsobed on protein carriers, and purified

Form :
Lyophilized

Tested applications :

Immunocytochemistry, ELISA. Optimal dilutions should be determined by each laboratory for each application.

Research areas :

Neurobiology

Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.

Specificity :

Using a conjugate Dopamine-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :

(a) : Dopamine-G-(Pc) concentration/unconjugated or conjugated catecholamine concentration at half displacement; G = Glutaraldehyde

Corresponding antigen :

Gemacbio sells the corresponding antigen : Dopamine(BSA) conjugate (code number : AG001)

References :

• GEFFARD M., BUIJS R.M., SEGUELA P., POOL C.W. and LE MOAL M. First demonstration of highly specific and sensitive antibodies against dopamine. Brain Res., (1984) 294, 161-165.
• GEFFARD M., SEGUELA P. and HEINRICH-ROCK A. M. Antisera against catecholamines : specificity studies and physicochemical data for anti-dopamine and anti-p-tyramine antibodies. Molecular Immunology, (1984) 21, 515-522.
• GEFFARD M., KAH O., ONTENIENTE B., SEGUELA P., LE MOAL M. and DELAAGE M.A. Antibodies to dopamine : radioimmunological study of specificity in relation to immunocytochemistry. J. Neurochem. (1984) 42, 1593-1599.
• KITAHAMA K., BUDA C., SASTRE J.P., NAGATSU I., RAYNAUD B., JOUVET M. and GEFFARD M. Dopaminergic neurons in the cat dorsal motor nucleus of the vagus, demonstrated by dopamine, AADC and TH immunohistochemistry. Neurosc. Letters, (1992), 146, 5-9.
• ARAI R., KARASAWA N., GEFFARD M., NAGATSU T., NAGATSU I. Immunohistochemical evidence that central serotonin neurons produce dopamine from exogenous L-DOPA in the rat, with reference to the involvement of aromatic L-amino acid decarboxylase. Brain research (1994) 667(2), 295-99.
• IKEMOTO K., SATOH K., KITAHAMA K., GEFFARD M. and MAEDA T. Electron-microscopic study of dopaminergic structures in the medial subdivision of the monkey nucleus accumbens. Brain research (1996) 111(1), 41-50.
• KITAHAMA K., NAGATSU I., GEFFARD M. and MAEDA T.Distribution of dopamine-immunoreactive fibers in the rat brainstem. Journal of Chemical Neuroanatomy 18(2000)1-9.
• TURCHAN J., ANDERSON C., HAUSER K.F., SUN Q., ZHANG J., LIU Y., WISE P.M., KRUMAN I., MARAGOS W., MATTSON M.P., BOOZE R. and NATH A. Estrogen protects against the synergistic toxicity by HIV proteins, methamphetamine and cocaine. BMC Neuroscience (2001) 2:3.
• XU J., KAO S.Y., LEE F.J.S., SONG W., JIN L.W. and YANKNER B.A. Dopamine-dependent neurotoxicity of α-synuclein : a mechanism for selective neurodegeneration in Parkinson disease. Nature Medicine (2002) 8(6), 600-606.
• FIORE G., POLI A., DI COSMO A., D’ISCHIA M. and PALUMBO A. Dopamine in the ink defence system of Sepia officinalis : biosynthesis, vesicular compartmentation in mature ink gland cells, nitric oxide (NO)/cGMP-induced depletion and fate in secreted ink. Biochem. J. (2004) 378, 785-91.
• KIM J, JEON M, PAENG KJ, PAENG IR. Competitive enzyme-linked immunosorbent assay for the determination of catecholamine, dopamine in serum. Anal Chim Acta. 2008 Jun 30;619(1):87-93.
• TINIKUL Y., MERCIER A.J. and SOBHON P. Distribution of dopamine and octopamine in the central nervous system and ovary during the ovarian maturation cycle of the giant freshwater prawn, Macrobrachium rosenbergii. Tissue and Cell (2009).
 

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