anti-conjugated Dopamine antibody
Product Number:
AM001
Host:
Mouse
Clonality:
Polyclonal
Target :
Conjugated Dopamine
Immunogens :
Synthetic Dopamine conjugated to protein carriers (Pc)
Quantity :
100µl
Isotype :
IgG 1, Kappa
Clone number :
HYDA 001
Purity :
previously preabsobed on protein carriers, and purified
Form :
Lyophilized
Tested applications :
Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application.
Research areas :
Neurobiology, Pharmacology , Biochemistry
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate Dopamine-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Dopamine-G-(Pc) | 1 |
| L-DOPA-G-(Pc) | 1/10,000 |
| L-Tyrosine-G-(Pc) | 1/36,000 |
| Tyramine-G-(Pc) | 1/> 50,000 |
| Noradrenaline-G-(Pc) | 1/> 50,000 |
| Octopamine-G-(Pc) | 1/> 50,000 |
| Adrenaline-G-(Pc) | 1/> 50,000 |
| free Dopamine | 1/> 50,000 |
(a) : Dopamine-G-(Pc) concentration/unconjugated or conjugated catecholamine concentration at half displacement, G = Glutaraldehyde
Corresponding antigen :
Gemacbio sells the corresponding antigen : Dopamine(BSA) conjugate (code number : AG001)
References :
• CHAGNAUD J.L., MONS N., TUFFET S., GRANDIER-VAZEILLES X. and GEFFARD M. Monoclonal antibodies against glutaraldehyde-conjugated dopamine. J. Neurochem., (1987) 49, 487-494 .
• GEFFARD M., TUFFET S., MONS N., CHAGNAUD JL. Simultaneous detection of indoleamines and dopamine in rat dorasal nucleus by using specific antibodies. Histochemistry (1987), 88, 61-64.
• DECAVEL C., LESCAUDRON L., MONS N. and CALAS A. First visualization of dopaminergic neurons with a monoclonal antibody to dopamine : a light and electron microscopic study. J. Histochem. Cytochem., (1987) 35, 1245-1252.
• SCHÜRMANN F.W., ELEKES K. and GEFFARD M. Dopamine-like immunoreactivity in the bee brain. Cell & Tissue Res., (1989), 256, 399-410.
• GOLDMAN-RAKIC P.S., LERANTH C., WILLIAMS S.M., MONS N. and GEFFARD M. Dopamine synaptic complex with pyramidal neurons in primate cerebral cortex. Proc. Nat. Acad. Sci. USA, (1989), 86, 9015-9019.
• DABADIE H., MONS N. and GEFFARD M. Simultaneous detection of tryptamine and dopamine in rat substantia nigra and raphe nuclei using specific antibodies. Brain Res., (1990) 512,138-142.,
• KITAHAMA K., GEFFARD M., OKAMURA H., NAGATSU I., MONS N. and JOUVET M. Dopamine and DOPA-immunoreactive neurons in the cat forebrain with reference to tyrosine hydroxylase-immunohistochemistry. Brain Res., (1990) 518, 83-94.
• KITAHAMA K., BUDA C., SASTRE J.P., NAGATSU I., RAYNAUD B., JOUVET M. and GEFFARD M. Dopaminergic neurons in the cat dorsal motor nucleus of the vagus, demonstrated by dopamine, AADC and TH immunohistochemistry. Neurosc. Letters, (1992) 146, 5-9.
• KITAHAMA K., IKEMOTO K., JOUVET A., NAGATSU I., GEFFARD M., OKAMURA H. and PEARSON J. Dopamine synthesizing enzymes in paraventricular hypothalamic neurons of the human and monkey (Macaca fuscata). Neurosci. Lett. (1998) 243, 1-4.
• KITAHAMA K., NAGATSU I., GEFFARD M., MAEDA T. Distribution of dopamine-immunoreactive fibers in the rat brainstem. J. Chem. Neuroanat. (2000) 18, 1-9.
• PARK I., KITAHAMA K., GEFFARD M. and MAEDA T. Posnatal development of the dopaminergic neurons in the rat mesencephalon. Brain and Development 22 (2000) S38-S44.
• MAEDA T, NAGATA K, YOSHIDA Y. Exogenous l-DOPA induce no dopamine immuno-reactivity in striatal astroglias and microglias of adult rats with extensive nigro-striatal dopaminergic denervation. Neurosci Lett. 2008 Mar 15;433(3):255-8.
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