anti-conjugated D.Alanine antibodies
Product Number:
AP091
Host:
Rabbit
Clonality:
Polyclonal
Target :
conjugated D-Alanine
Immunogens :
Synthetic D-Alanine conjugated to Protein Carriers (PC).
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and ammonium sulphate purified
Form :
Lyophilized
Tested application :
Immunohistochemistry. Optimal dilutions should be determined by each laboratory for each application.
Research areas :
Neuroscience (neuromodulators), Biochemistry (stereochemistry studies).
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate D-Alanine-Glutaraldehyde-PC, antibody specificity was performed by competitive
ELISA with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| D-Alanine-G-PC | 1 |
| L-Alanine-G-PC | 1/> 50,000 |
| Glycine-G-PC | 1/> 50,000 |
| D-Valine-G-PC | 1/> 50,000 |
| D-Leucine-G-PC | 1/> 50,000 |
(a) : D-Alanine-G-PC concentration/ Other conjugated aminoacids concentration at half displacement.
G = Glutaraldehyde.
Corresponding antigen :
Gemacbio sells the corresponding antigen: D-Alanine(BSA) conjugate (code number: AG091)
Reference :
KIM HG, KIM NR, GIM MG, LEE JM, LEE SY, KO MY, KIM JY, HAN SH, CHUNG DK. Lipoteichoic acid isolated from Lactobacillus plantarum inhibits lipopolysaccharide-induced TNF-alpha production in THP-1 cells and endotoxin shock in mice. J. Immunol. (2008) 180(4):2553-61.
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com
