anti-conjugated Acrolein antibodies
Product Number:
AP051
Host:
Rabbit
Clonality:
Polyclonal
Target :
Conjugated Acrolein
Immunogens :
Synthetic Acrolein conjugated to Protein carriers
Quantity :
100µl
Isotype :
IgG
Purity :
Antiserum previously preabsobed on protein carriers, and purified
Form :
Lyophilized
Tested applications :
Immunohistochemistry, Immunoblot. Optimal dilutions should be determined by each laboratory for each application
Research areas :
Neuroscience, oxidative processes, biochemistry (metabolic ways)
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate Acrolein-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity (a) |
|---|---|
| Acrolein-BSA | 1 |
| Malondialdehyde-BSA | 1/55 |
| Glyoxal-BSA | 1/>50,000 |
| Formaldehyde-BSA | 1/>50,000 |
| Glutaraldehyde-BSA | 1/>50,000 |
(a) : Acrolein-BSA concentration /Conjugated closed related compounds concentration at half displacement.
BSA = Bovine Serum Albumin.
Corresponding antigen :
Gemacbio sells the corresponding antigen: Acrolein(BSA) conjugate (code number: AG051)
References :
• SHEN J., YANG X., DONG A., PETTERS R.M., PENG Y-W, WONG F. and CAMPOCHIARO P.A. Oxidative damage is a potential cause of cone cell death in retinitis pigmentosa. J. of Cellular Physiology (2005) 203, 457-464.
• KOMEIMA K, ROGERS BS, LU L, CAMPOCHIARO PA. Antioxidants reduce cone cell death in a model of retinitis pigmentosa. Proc Natl Acad Sci U S A. (2006) 103(30), 11300-05.
• HAMANN K, DURKES A, OUYANG H, UCHIDA K, POND A, SHI R. Critical role of acrolein in secondary injury following ex vivo spinal cord trauma. J Neurochem. (2008) 107(3), 712-21.
• DORRELL MI, AGUILAR E, JACOBSON R, YANES O, GARIANO R, HECKENLIVELY J, BANIN E, RAMIREZ GA, GASMI M, BIRD A, SIUZDAK G and FRIEDLANDER M. Antioxidant or neurotrophic factor treatment preserves function in a mouse model of neovascularization-associated oxidative stress. The Journal of Clinical Investigation (2009) 119(3), 611-623.
• DAIKI R, NISHIMURA K, ISHII I, OMURA T, OKUYAMA S, KASHIWAGI K and IGARASHI K Intense correlation between brain infarction and protein-conjugated acrolein. Stroke (2009) 40, 1-6.
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com
