anti-conjugated Acetylcholine antibody
Product Number:
AM002
Host:
Mouse
Clonality:
Monoclonal
Target :
Conjugated Acetylcholine
Immunogens :
Synthetic choline-glutaric acid conjugated to protein carriers
Quantity :
100µl
Isotype :
IgG2a, Kappa
Clone number :
HYChol001
Purity :
previously preabsobed on protein carriers, and purified
Form :
Lyophilized
Tested applications :
Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application
Research areas :
Neurobiology, Pharmacology, Biochemistry, Degenerative diseases
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate choline-glutaric acid-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Choline-GA-(Pc) | 1 |
| Choline-AA-(Pc) | 1/500 |
| Choline-SA-(Pc) | 1/2,500 |
| Farnesol-(Pc) | 1/> 50,000 |
| Retinoic acid-(Pc) | 1/> 50,000 |
| Myristic acid-(Pc) | 1/> 50,000 |
| free Acetylcholine | 1/> 50,000 |
| free Choline | 1/> 50,000 |
(a) : Choline-GA-(Pc) concentration/unconjugated or conjugated compounds concentration at half displacement ;
GA = Glutaric acid, AA = Adipic acid, SA = Succinic acid
References :
• CHAGNAUD J.L., SOUAN M.L., CHARRIER M.C. and GEFFARD M. Monoclonal anti-conjugated acetylcholine antibody and immunohistochemical applications in rat nervous system. J. Neurochem. (1989) 53, 383-391.
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