anti-conjugated Acetylcholine antibodies
Product Number:
AP049
Host:
Rabbit
Clonality:
Polyclonal
Target :
Conjugated Acetylcholine
Immunogens :
Synthetic choline-glutaric acid conjugated to protein carriers
Quantity :
100µl
Isotype :
IgG
Purity :
previously preabsobed on protein carriers, and purified
Form :
Lyophilized
Tested applications :
Immunocytochemistry. Optimal dilutions should be determined by each laboratory for each application
Research areas :
Neurobiology, Pharmacology, Biochemistry, Degenerative diseases
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate choline-glutaric acid-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| Choline-GA-PL | 1 |
| Choline-allyl alcohol-G-BSA | 1/2 |
| Phosphatidylcholine | 1/>10,000 |
| free Choline | 1/>10,000 |
| free Acetylcholine | 1/>10,000 |
(a) : Choline-GA-BSA concentration /unconjugated or conjugated analogs concentration at half displacement.
GA = Glutaric anhydride
BSA = Bovine Serum Albumin
References :
• GEFFARD M., VIEILLEMARINGE J., HEINRICH-ROCK A.M. and DURIS P. Anti-acetylcholine antibodies and first immunocytochemical application in insect brain. Neurosci. Lett., (1985) 57, 1-6.
• GEFFARD M., Mc RAE-DEGUEURCE A. and SOUAN M.L. Immunocytochemical detection of acetylcholine in the rat central nervous system. Science, (1985) 229, 77-79.
• Mc RAE-DEGUEURCE A. and GEFFARD M. One perfusion mixture for immunocytochemical detection of noradrenaline, dopamine, serotonin and acetylcholine in the same rat brain. Brain Res., (1986) 376, 217-219.
• WANG-BENNETT L.T., SOUAN M.L. and GLANTZ R.M. Immunocytochemical studies of the distribution of acetylcholine in the crayfish. J. Comp. Neurol., (1988) 273, 330-343.
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