anti-conjugated 5-dihydroxytryptophan antibody
Product Number:
AM006
Host:
Mouse
Clonality:
Monoclonal
Target :
Conjugated DL-5-Hydroxytryptophan
Immunogens :
Synthetic DL-5-Hydroxytryptophan conjugated to protein carriers (Pc)
Quantity :
100µl
Isotype :
IgG1, Kappa
Clone number :
HHW1
Purity :
previously preabsobed on protein carriers, and purified
Form :
Lyophilized
Tested applications :
Immuno-histochemistry. Optimal dilutions should be determined by each laboratory for each application
Research areas :
Neurobiology, Pharmacology, Biochemistry, Neurodegenerative diseases
Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.
Specificity :
Using a conjugate DL-5-Hydroxytryptophan-BSA, antibody specificity was performed with an ELISA test by competition experiments with the following compounds :
| Compounds | Cross-reactivity ratio (a) |
|---|---|
| DL.5-Hydroxytryptophan-G-BSA | 1 |
| L-Tryptophan-G-BSA | 1/>10,000 |
| 5-Methoxytryptophan-G-BSA | 1/>10,000 |
(a) : DL-5-Hydroxytryptophan-G-BSA concentration/other conjugated indolamines concentration at half-displacement ;
G = Glutaraldehyde, BSA = Bovine Serum Albumin.
Corresponding antigen :
Gemacbio sells the corresponding antigen : 5-Hydroxytryptophan conjugate (code number : AG016)
References :
• TOURET M., KITAHAMA K., GEFFARD M. and JOUVET M. 5-hydroxytryptophan (5-HTP)-immunoreactive neurons in the rat brain tissue. Neurosc. Letters, (1987) 80, 263-267.
• TOURET M., SARDA N., GHARIB A., GEFFARD M. and JOUVET M. The role of 5-hydroxytryptophan (5-HTP) in the regulation of the sleep/wake cycle in parachlorophenylalanine (p-CPA) pretreated rat : a multiple approach study. Exp. Brain Res., (1991) 86, 117-124.
For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com
