anti-conjugated 5-dihydroxytryptophan antibodies

Product Number: 
AP016
Host: 
Rabbit
Clonality: 
Polyclonal
Gemacbio

Target :

Conjugated 5-Hydroxytryptophan


Immunogens :

Synthetic 5-Hydroxytryptophan conjugated to protein carriers (Pc)


Quantity :
100µl


Isotype :
IgG


Purity :
previously preabsobed on protein carriers, and purified


Form :
Lyophilized


Tested applications :
Immuno-histochemistry. Optimal dilutions should be determined by each laboratory for each application


Research areas :

Neurobiology, Pharmacology, Biochemistry, Neurodegenerative diseases


Storage instructions :
Lyophilized vial must be stored at 4°C in a dry area. After reconstitution with 50μl of distilled water and 50μl of glycerol, the aliquot can be stored at -20°C, and is stable at least 2 years.


Specificity :

Using a conjugate 5-Hydroxytryptophan-(Pc), antibody specificity was performed with an ELISA test by competition experiments with the following compounds :

          Compounds         Cross-reactivity ratio (a)
5-Hydroxytryptophan-G-(Pc) 1
5-Methoxytryptophan-G-(Pc)  1/2,000
L-Tryptophan-G-(Pc)  1/50,000
5-Hydroxytryptamine-G-(Pc)  1/50,000
Tryptamine-G-(Pc)  1/>50,000
free 5-Hydroxytryptophan 1/>50,000

(a) : 5-Hydroxytryptophan-G-(Pc) concentration/other conjugated indolealkylamines concentration at half displacement ; G = Glutaraldehyde


Corresponding antigen :

Gemacbio sells the corresponding antigen : 5-Hydroxytryptophan conjugate (code number : AG016)


References :

• GEFFARD M., TOURET M. and KITAHAMA K. First characterization of 5-hydroxytryptophan in rat brain by using specific antibodies, Brain Res., (1987), 426, 191-196.
• TOURET M., KITAHAMA K., GEFFARD M. and JOUVET M. 5-hydroxytryptophan (5-HTP)-immunoreactive neurons in the rat brain tissue. Neurosc. Letters, (1987) 80, 263-267.
• TOURET M., SARDA N., GHARIB A., GEFFARD M. and JOUVET M. The role of 5-hydroxytryptophan (5-HTP) in the regulation of the sleep/wake cycle in parachlorophenylalanine (p-CPA) pretreated rat : a multiple approach study. Exp. Brain Res., (1991) 86, 117-124.
 


For all questions about price and availability of this product, please send us an email specifying your needs. We will immediately send you back a detailed estimate. ab [at] gemacbio [dot] com

 

 

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